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Immunoprecipitation of main WT1 isoforms with C-19 antibody but not with handle non-immune IgG was confirmed. However,PLOS 1 | DOI:ten.1371/journal.pone.0130578 June 19,10 /Novel WT1 Spliced IsoformEx4a(+)WT1 isoform wasn't co-precipitated with significant WT1 isoform making use of C-19 antibody (Figure B in S2 Fig). Consequently, it appeared that Ex4a(+)WT1 isoform didn't physically affiliate wit