Licell CM, Millipore), was placed on a flow chamber and hugely purified bone marrow derived PMNs had been perfused over the pMBMECs. PMNs had been permitted to accumulate for 4 min at low shear pressure (0.25 dyn/cm2); the subsequent PMN interaction with pMBMECs under physiological shear strain (1.five dyn/cm2) was recorded with an inverted microscope (AxioObserver Z1, Carl Zeiss) at 209 magnifica