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R two min in EDTA answer and trypsinized for 2 min in 500 l of a 0.25 trypsin option. Human melanoma cells. The M21 melanoma cell line was obtained from the American Sort Culture Collection (ATCC, Manassas, VA), and maintained in RPMI containing 10 FBS and 1 PenStrep.Microarrays and qRT-PCRZebrafish. tfap2a-/- mutant [lockjaw allele, 28] and non-mutant siblings (i.e. wildtype and tfap2a+/- hete