00 cells/well) in 6-well tissue culture plates had been infected with recombinant adenoviruses within the absence (EtOH) or presence of 10-9 M E2. 24h soon after infection, more fresh medium containing two.5 M EdU, without the need of (EtOH) or with 10-9 M E2, had been added and cells had been additional maintained for 24h. Cells were then harvested by trypsinization, fixed with Click-IT fixative,